QCM Chemotaxis Cell Migration Assay, 24-well (8 µm), colorimetric

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Seed 2×105 cells in the top chamber	Cover plate and incubate for 24 to 36 h hours at 37°C in a CO2 incubator (4-6% CO2).

Upstream tips
Seed 2×105 cells in the top chamber
Protocol tips
Cover plate and incubate for 24 to 36 h hours at 37°C in a CO2 incubator (4-6% CO2).

Publication protocol

HUVECs were transfected with 50 nM FGF21 mimics, FGF21 inhibitor, or control plasmid. To evaluate cells migration 24 h after transfection, 2×105 cells were seeded in the top chamber of the Transwell insert containing the non-coated membrane. To evaluate cells invasion 24 h after transfection, 5×104 cells were seeded in the top chamber of the Transwell insert containing a membrane coated with Matrigel. In both assays, the top chamber contained serum-free DMEM, and the bottom chamber contained DMEM supplemented with 10% FBS as a chemoattractant. The plates were incubated for 24 to 36 h, and cells remaining on the top of the membrane were removed by a cotton swab. Migration and invasion were assessed using the QCMTM 24-Well Colorimetric Cell Migration Assay Kit according to the manufacturer’s instructions [20]. Cells on the underside of the membrane were counted at ×100 magnification in at least three randomly selected fields.

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Manufacturer protocol

Download the product protocol from Merck Millipore for QCM Chemotaxis Cell Migration Assay, 24-well (8 µm), colorimetric below.

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