QCM ECMatrix Cell Invasion Assay, 24-well (8 µm), colorimetric

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Do not freeze.	Incubate 48 hours in a culture incubator. Stain invasive cells on lower surface of the membrane by dipping inserts in the staining solution for 20 minutes.

Upstream tips
Do not freeze.
Protocol tips
Incubate 48 hours in a culture incubator. Stain invasive cells on lower surface of the membrane by dipping inserts in the staining solution for 20 minutes.

Publication protocol

For assessment of cell motility, chamber migration assays were conducted using a cell culture insert (8-µm pore size, 24-well format; Cell Invasion Assay Kit Cat. CHEMICON, No. ECM550). A549-negative control and A549-BCAR1-RNAi cells were seeded in duplicate at a density of 3.0×105 cells/chamber. After 48 hours, cells which had not moved to the lower wells were removed from the upper face of the filters using cotton swabs, and cells that had moved to the lower surface of the filter were stained by using a Cell Invasion Assay Kit Cat. (CHEMICON, No. ECM550). Cell migration was quantified by visual counting after being photographed. Experiments were performed in duplicate. Mean values for three random fields were obtained for each well.

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