CytoSelect™ 24-Well Cell Migration and Invasion Assay Combo Kit, 8 µm

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Suspend 100 000 cells/mL	Incubate for 72 hours in a cell culture incubator.

Upstream tips
Suspend 100 000 cells/mL
Protocol tips
Incubate for 72 hours in a cell culture incubator.

Publication protocol

Cell invasion was measured using the Cell CytoSelect 24-Well Cell Migration and Invasion Assay Combo kit, 8 μm (fluorometric quantitation) (Cell Biolabs) following the manufacturer's protocol. Briefly, cell suspensions containing 100 000 cells/mL (in serum-free medium), which had been treated with or without 1nM R1881, were transfected TM4SF3 siRNA (3′UTR specific) or control siRNA in the absence or presence of TM4SF3 adenovirus (hTSPAN8-His-adenovirus from Abcam). LNCaP, C81, and PC-3 cells were infected with 20 multiplicity of infection of virus. Cells were used to monitor cell invasion into a lower chamber containing RPMI 1640 medium with 10% DCC-stripped serum. After 72 hours of incubation at 37°C, cells were quantified by measuring fluorescence using a microplate reader.

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Manufacturer protocol

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