Oris™ Cell Migration Assay - Fibronectin Coated

Protocol tips

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	Incubate the seeded plate containing the Oris™ Cell Seeding Stoppers in a humidified chamber (37°C, 5% CO2) for 24 hours to permit cell attachment.

Protocol tips
Incubate the seeded plate containing the Oris™ Cell Seeding Stoppers in a humidified chamber (37°C, 5% CO2) for 24 hours to permit cell attachment.

Publication protocol

Chemostatic cell movement was analyzed using an Oris™ cell migration assay kit (Platypus Technologies, Madison, WI) as described previously [48]. For the chemotactic cell migration assay, a Transwell-chamber insert (Corning Costar, Cambridge, MA) with filters precoated on the lower surface with gelatin was placed in a 24-well tissue culture plate containing conditioned medium from NIH3T3 fibroblasts cultured in complete medium. Cells (2 × 104) suspended in serum-free RPMI medium were added to the upper chambers. Following a 24 hour incubation, cells were fixed and stained with crystal violet. After removal of non-migrated cells on the upper surface of the filter, chemotactic cell motility was quantified by counting the cells on the lower surface of the filter

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