Cultrex® Cell Migration Assay

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	Incubate cells at 37 oC in a CO2 incubator for 24 hours

Protocol tips
Incubate cells at 37 oC in a CO2 incubator for 24 hours

Publication protocol

Cell migration was measured using the Cultrex cell migration assay (Trevigen). Briefly, cells were plated in the upper chamber of a 24-well Transwell plate. The lower chamber contained DMEM medium with 10% (vol/vol) FBS. After 24 h, the cells were collected in a cell-dissociation solution containing 1 μM of Calcein-AM. Percentages of migrated cells were calculated from the standard curve established for respective cell lines.

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