Cultrex® Cell Migration Assay

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	Incubate for 16 hours at 37ºC in 5% CO2 atmosphere

Protocol tips
Incubate for 16 hours at 37ºC in 5% CO2 atmosphere

Publication protocol

The cell migration assays were described previously (19). Briefly, after transfection, MHCC97H cells were serum-starved for 24 h, and 5 × 104 cells were seeded on Transwell inserts (8-μm pore size, Cultrex 96-well cell migration assay, catalog no. 3465-096-K, Trevigen). After 16 h, the non-migrated cells were removed from the insert with a cotton swab. The migrated cells were fixed for 10 min in 3.7% (v/v) formaldehyde in PBS before staining with 0.1% crystal violet for 15 min, followed by washing with PBS. Images were taken with an Olympus MicroFire/Qcam CCD 1 × 81 microscope. The crystal violet-stained cells were counted. Cell invasion assays were performed as described for the migration assays, except that cells were seeded on precoated inserts with basement membrane extract solution (Cultrex 96-well BME cell invasion assay, catalog no. 3455-096-K, Trevigen). Invading cells were fixed and stained. Images were taken, and cells were counted.

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