CytoSelect™ 24-Well Cell Migration and Invasion Assay Combo Kit, 8 µm

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Suspend 100 000 cells/mL	Incubate for 72 hours in a cell culture incubator.

Upstream tips
Suspend 100 000 cells/mL
Protocol tips
Incubate for 72 hours in a cell culture incubator.

Publication protocol

Cell invasion was measured using the Cell CytoSelect 24-Well Cell Migration and Invasion Assay Combo kit, 8 μm (fluorometric quantitation) (Cell Biolabs) following the manufacturer's protocol. Briefly, cell suspensions containing 100 000 cells/mL (in serum-free medium), which had been treated with or without 1nM R1881, were transfected TM4SF3 siRNA (3′UTR specific) or control siRNA in the absence or presence of TM4SF3 adenovirus (hTSPAN8-His-adenovirus from Abcam). LNCaP, C81, and PC-3 cells were infected with 20 multiplicity of infection of virus. Cells were used to monitor cell invasion into a lower chamber containing RPMI 1640 medium with 10% DCC-stripped serum. After 72 hours of incubation at 37°C, cells were quantified by measuring fluorescence using a microplate reader.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Cell migration / Invasion cell type - PC-3 using CytoSelect™ 24-Well Cell Migration and Invasion Assay Combo Kit, 8 µm from Cell Biolabs.

Manufacturer protocol

Download the product protocol from Cell Biolabs for CytoSelect™ 24-Well Cell Migration and Invasion Assay Combo Kit, 8 µm below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Cell migration / Invasion cell type - PC-3 using CytoSelect™ 24-Well Cell Migration and Invasion Assay Combo Kit, 8 µm from Cell Biolabs. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.