QCM ECMatrix Cell Invasion Assay, 24-well (8 µm), fluorimetric

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Starve cells by incubating 18-24 hours prior to assay in appropriate serumfree medium (DMEM, EMEM, or equivalent).	Cover plate and incubate for 24 - 72 hours at 37°C in a CO2 incubator (4- 6% CO2).

Upstream tips
Starve cells by incubating 18-24 hours prior to assay in appropriate serumfree medium (DMEM, EMEM, or equivalent).
Protocol tips
Cover plate and incubate for 24 - 72 hours at 37°C in a CO2 incubator (4- 6% CO2).

Publication protocol

The cell invasion assay was performed with QCM 24-well Invasion Assay kit (Chemicon International). This cell invasion assay was performed in an invasion chamber, based on the Boyden chamber principle. This kit contains 24 inserts and each insert contains an 8-μm pore size polycarbonate membrane coated with a thin layer of ECM. The method was described previously [10].

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Manufacturer protocol

Download the product protocol from Merck Millipore for QCM ECMatrix Cell Invasion Assay, 24-well (8 µm), fluorimetric below.

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