Cultrex® Cell Migration Assay

Cell migration / Invasion cell type - MHCC-97H

Experiment
Cell migration / Invasion cell type - MHCC-97H
Product
Cultrex® Cell Migration Assay from Trevigen
Manufacturer
Trevigen

Protocol tips

Protocol tips
Incubate for 16 hours at 37ºC in 5% CO2 atmosphere

Publication protocol

The cell migration assays were described previously (19). Briefly, after transfection, MHCC97H cells were serum-starved for 24 h, and 5 × 104 cells were seeded on Transwell inserts (8-μm pore size, Cultrex 96-well cell migration assay, catalog no. 3465-096-K, Trevigen). After 16 h, the non-migrated cells were removed from the insert with a cotton swab. The migrated cells were fixed for 10 min in 3.7% (v/v) formaldehyde in PBS before staining with 0.1% crystal violet for 15 min, followed by washing with PBS. Images were taken with an Olympus MicroFire/Qcam CCD 1 × 81 microscope. The crystal violet-stained cells were counted. Cell invasion assays were performed as described for the migration assays, except that cells were seeded on precoated inserts with basement membrane extract solution (Cultrex 96-well BME cell invasion assay, catalog no. 3455-096-K, Trevigen). Invading cells were fixed and stained. Images were taken, and cells were counted.

Full paper   Login or join for free to view the full paper.

Reviews

Cultrex® Cell Migration Assay from Trevigen has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Start your discussion

Share your thoughts or question with experts in your field

Start a discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Cell migration / Invasion cell type - MHCC-97H using Cultrex® Cell Migration Assay from Trevigen.

Paper title
MicroRNA-433 Inhibits Liver Cancer Cell Migration by Repressing the Protein Expression and Function of cAMP Response Element-binding Protein
Full paper
Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from Trevigen for Cultrex® Cell Migration Assay below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Cell migration / Invasion cell type - MHCC-97H using Cultrex® Cell Migration Assay from Trevigen. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Become shareholder Discussions About us Contact Privacy Terms