QCM ECMatrix Cell Invasion Assay, 96-well (8 µm), fluorimetric

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Seed ~1×10^5 cells	Add 20% fetal bovine serum for lower chamber Cover plate and incubate for 12 - 24 hours at 37°C in a CO2 incubator (4-6% CO2).

Upstream tips
Seed ~1×10^5 cells
Protocol tips
Add 20% fetal bovine serum for lower chamber Cover plate and incubate for 12 - 24 hours at 37°C in a CO2 incubator (4-6% CO2).

Publication protocol

A novel quantitative transwell chamber with the Matrigel gel kit - QCM™ 96-well Cell Invasion Assay kit from Millipore (Billerica, MA, USA) was used to detect the invasion capacity of tumor cells, according to the manufacturer’s protocol. Briefly, ~1×105 tumor cells were plated into the upper chamber in serum-free medium and medium with 20% FBS was added into the lower chamber. After 24 h, cells under the invasion chamber were dislodged completely by the cell detachment solution and added into the lysis buffer/dye solution mixture. The results were obtained with a fluorescence plate reader using a 480/520-nm filter set. The experiments were performed with at least six replicates.

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Manufacturer protocol

Download the product protocol from Merck Millipore for QCM ECMatrix Cell Invasion Assay, 96-well (8 µm), fluorimetric below.

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