QCM ECMatrix Cell Invasion Assay, 24-well (8 µm), fluorimetric

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Add 20% fetal bovine serum to the lower chamber. Incubate for 24 - 72 hours at 37°C in a CO2 incubator

Protocol tips
Add 20% fetal bovine serum to the lower chamber. Incubate for 24 - 72 hours at 37°C in a CO2 incubator

Publication protocol

Cells were incubated with 10 μm BMVC or BMVC4 for 4 days, and the invasion assays were then performed using the QCM ECMatrix Cell Invasion assay kit (Millipore, ECM554). Briefly, ∼2 × 104 of the drug-treated cells were grown in the upper chamber in serum-free medium with 20% serum medium in the bottom chamber. Invasive migration of cells through the ECM layer was then determined by crystal violet staining of the bottom of upper chamber. The relative invasion activities were obtained by measurement of A560 and using the value of solvent control as 1.

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