QCM ECMatrix Cell Invasion Assay, 24-well (8 µm), fluorimetric

Cell migration / Invasion cell type - H1299

Experiment
Cell migration / Invasion cell type - H1299
Product
QCM ECMatrix Cell Invasion Assay, 24-well (8 µm), fluorimetric from Merck Millipore
Manufacturer
Merck Millipore

Protocol tips

Protocol tips
Add 20% fetal bovine serum to the lower chamber.

Incubate for 24 - 72 hours at 37°C in a CO2 incubator

Publication protocol


Cells were incubated with 10 μm BMVC or BMVC4 for 4 days, and the invasion assays were then performed using the QCM ECMatrix Cell Invasion assay kit (Millipore, ECM554). Briefly, ∼2 × 104 of the drug-treated cells were grown in the upper chamber in serum-free medium with 20% serum medium in the bottom chamber. Invasive migration of cells through the ECM layer was then determined by crystal violet staining of the bottom of upper chamber. The relative invasion activities were obtained by measurement of A560 and using the value of solvent control as 1.

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Cell migration / Invasion cell type - H1299 using QCM ECMatrix Cell Invasion Assay, 24-well (8 µm), fluorimetric from Merck Millipore.

Paper title
Inhibition of Cancer Cell Migration and Invasion through Suppressing the Wnt1-mediating Signal Pathway by G-quadruplex Structure Stabilizers
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Manufacturer protocol

Download the product protocol from Merck Millipore for QCM ECMatrix Cell Invasion Assay, 24-well (8 µm), fluorimetric below.

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