ORIS™ Cell Migration Assay

Protocol tips

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	Incubate the seeded plate containing the Oris™ Cell Seeding Stoppers in a humidified chamber (37°C, 5% CO2) for 4 to 18 hours (cell line dependent) to permit cell attachment.

Protocol tips
Incubate the seeded plate containing the Oris™ Cell Seeding Stoppers in a humidified chamber (37°C, 5% CO2) for 4 to 18 hours (cell line dependent) to permit cell attachment.

Publication protocol

Cell viability was evaluated with the Cell Titer GLO (Promega) according to the manufacturer protocol.

Wound healing ability was evaluated with the Oris Cell Migration Assay (Platypus Technologies) according to manufacturer protocol. Two days after the seeding, cells were fixed with 11% glutaraldehyde and stained with 0.1% crystal violet. Pictures were taken at 5× magnification, with a Leica DMIL microscope connected to a Leica DFC320 camera. The area of the hole was calculated with ImageJ software.

Soft agar, scatter and migration assays were conducted as in refs. 30, 34. Every biological assay was done at least 3 times.


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Manufacturer protocol

Download the product protocol from Platypus Technologies for ORIS™ Cell Migration Assay below.

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