Oris™ Cell Migration Assay - Collagen I Coated

Protocol tips

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	Incubate the seeded plate containing the Oris™ Cell Seeding Stoppers in a humidified chamber (37°C, 5% CO2) for 12 or 24 hours to permit cell attachment.

Protocol tips
Incubate the seeded plate containing the Oris™ Cell Seeding Stoppers in a humidified chamber (37°C, 5% CO2) for 12 or 24 hours to permit cell attachment.

Publication protocol

The Oris™ cell migration assay kit (collagen I coated) was used according to the manufacturer's protocol (CMACC5.101, Platypus Technologies LLC, Madison, WI, USA). HaCaT cells were seeded at a density of 5 × 104 cells per well in an Oris 96-well migration assay plate with cell seeding stoppers. Cells were cultured for 24 hours. The cell stoppers were removed, and 100 μL of fresh medium with or without MTOR inhibitors was replaced, followed by incubation for 12 or 24 hours. The cell migration to the zone isolated by stoppers was observed, and the micrographs were captured under inverted microscopy. Cells in the migration zone were replicated for five independent experiments. The areas without cell migration per well were measured. The cell migration parameter was calculated using the following formula: (100%  −  areas without cell migration/area isolated by stopper) × 100

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Manufacturer protocol

Download the product protocol from Platypus Technologies for Oris™ Cell Migration Assay - Collagen I Coated below.

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