Protocol tips
Upstream tips |
Protocol tips |
Downstream tips |
Prepare a cell suspension containing 0.5-1.0 x 106 cells/ml in serum free media |
Incubate for 2-24 hours in a cell culture incubator. |
Read fluorescence with a fluorescence plate reader at 480 nm/520 nm. |
Upstream tips |
Prepare a cell suspension containing 0.5-1.0 x 106 cells/ml in serum free media |
Protocol tips |
Incubate for 2-24 hours in a cell culture incubator. |
Downstream tips |
Read fluorescence with a fluorescence plate reader at 480 nm/520 nm. |
Publication protocol
The transwell migration and invasion assay was performed using the Cytoselect 24-well cell Migration and Invasion Assay kit (Cell Biolabs, San Diego, CA, USA) according to the manufacturer’s instruction
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Papers
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Paper title
The metastatic suppressor NDRG1 inhibits EMT, migration and invasion through interaction and promotion of caveolin-1 ubiquitylation in human colorectal cancer cells
Manufacturer protocol
Download the product protocol from Cell Biolabs for CytoSelect™ 24-Well Cell Migration Assay, 8 μm below.
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