QCM™ Tumor Cell Transendothelial Migration Assay (Colorimetric, 24 Assays)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Add 1x10^5 endothelial cells	Incubate 24 hrs in the cell incubator.	Read plate at absorbance 540 – 570 nm

Upstream tips
Add 1x10^5 endothelial cells
Protocol tips
Incubate 24 hrs in the cell incubator.
Downstream tips
Read plate at absorbance 540 – 570 nm

Publication protocol

Subsequent to transfection of the SW480 and HCT116 cells with miR-100 mimics, antisense or NC for 24 h, cell migration and invasion were analyzed. The cells were seeded in Transwell migration or extracellular matrix-coated invasion chambers (Tumor Cell Transendothelial Migration Assay kit and ECMatrix Cell Invasion Assay kit, respectively; EMD Millipore, Temecula, CA, USA) and incubated for a further 24 h. Subsequently, the Transwell migration assay or invasion assay was conducted according to manufacturer’s instructions. Cell migration and invasion were quantified with an iMark Microplate Absorbance Reader (#168–1130; Bio-Rad Laboratories, Hercules, CA, USA) at 570 nm, according to the manufacturer’s instructions.

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Manufacturer protocol

Download the product protocol from Merck Millipore for QCM™ Tumor Cell Transendothelial Migration Assay (Colorimetric, 24 Assays) below.

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