QCM ECMatrix Cell Invasion Assay, 24-well (8 µm), fluorimetric

Protocol tips

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	Add 10% fetal bovine serum) to the lower chamber. Cover plate and incubate for 24 - 72 hours at 37°C in a CO2 incubator (4-6% CO2).

Protocol tips
Add 10% fetal bovine serum) to the lower chamber. Cover plate and incubate for 24 - 72 hours at 37°C in a CO2 incubator (4-6% CO2).

Publication protocol

In vitro cell invasion assays were performed with the QCM ECMatrix 24-well (8 µM) Fluorimetric Cell Invasion Assay kit (Chemicon; Millipore) according to the manufacturer's instructions [19], [20]. The kit used an insert polycarbonate membrane with an 8 µM pore size. The insert in the invasion kit was coated with a thin layer of ECMatrix. Cell invasion was determined by fluorescence. Each experiment was repeated for three times in duplicates.

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Manufacturer protocol

Download the product protocol from Merck Millipore for QCM ECMatrix Cell Invasion Assay, 24-well (8 µm), fluorimetric below.

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