CytoSelect™ 24-Well Cell Migration Assay, 5 µm

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Seed 4 × 10^4 cells	Incubate cells at 37 °C for 24 hours	Read plate at 485/520 nm filter set.

Upstream tips
Seed 4 × 10^4 cells
Protocol tips
Incubate cells at 37 °C for 24 hours
Downstream tips
Read plate at 485/520 nm filter set.

Publication protocol

The transwell cell migration assay was performed using a Fluorometric Cell Migration Assay kit with polycarbonate membrane inserts (5µm pore size; Cell Biolabs, San Diego, CA, USA). Cells were serum-starved overnight in DMEM prior to initiation of the experiment. The lower chambers were filled with 1 ml of conditioned media. Cells (4 × 104) were re-suspended in 200µl of DMEM and added to the upper chamber. Cells were then incubated at 37 °C for 24 hours to allow cell migration through the membrane. Migratory cells were detached from the underside of the membrane and subsequently lysed and detected by CyQuant GR dye (Invitrogen). Fluorescence measurement was performed in a FluoStar Optima fluorescence plate reader with a 485/520 nm filter set.

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Manufacturer protocol

Download the product protocol from Cell Biolabs for CytoSelect™ 24-Well Cell Migration Assay, 5 µm below.

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