24-Well Cell Invasion Assays, Basement Membrane

Cell migration / Invasion cell type - A375

Experiment
Cell migration / Invasion cell type - A375
Product
24-Well Cell Invasion Assays, Basement Membrane from Cell Biolabs
Manufacturer
Cell Biolabs

Protocol tips

Upstream tips
Store all components at 4ºC and before use allow the invasion chamber plate to warm up at room temperature for 10 minutes.
Protocol tips
Incubate for 72 hours at 37ºC in 5% CO2 atmosphere.
Downstream tips
Measure the OD 570nm in a
plate reader

Publication protocol

In vitro cell invasion assay was performed using a basement membrane-coated CytoSelect™ 24-well Cell Invasion Assay Kit (8 mm pores, Colorimetric Format, Cell Biolabs, San Diego, CA, US) according to the manufacturer’s instruction. Briefly, CD10- or mock-A375 cells were serum-starved for 24 h. Next, the cells suspended with serum-free media (3 X 105 cells/well) were seeded in the upper chamber separated from a lower chamber filled with media containing 10% FBS and incubated for 72 h. After noninvasive cells were removed, the invasive cells were stained and quantified by measuring the absorbance at 570 nm using a microplate reader (iMark™ Microplate Absorbance Reader, Bio-Rad Laboratories). All assays were carried out 3 times in triplicates.

Full paper   Login or join for free to view the full paper.

Reviews

24-Well Cell Invasion Assays, Basement Membrane from Cell Biolabs has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Start your discussion

Share your thoughts or question with experts in your field

Start a discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Cell migration / Invasion cell type - A375 using 24-Well Cell Invasion Assays, Basement Membrane from Cell Biolabs.

Paper title
CD10-Equipped Melanoma Cells Acquire Highly Potent Tumorigenic Activity: A Plausible Explanation of Their Significance for a Poor Prognosis
Full paper
Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from Cell Biolabs for 24-Well Cell Invasion Assays, Basement Membrane below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Cell migration / Invasion cell type - A375 using 24-Well Cell Invasion Assays, Basement Membrane from Cell Biolabs. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Become shareholder Discussions About us Contact Privacy Terms