96-Well Cell Invasion Assay, Basement Membrane

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	Cover the plate and transfer to a cell culture incubator for 24 hours

Protocol tips
Cover the plate and transfer to a cell culture incubator for 24 hours

Publication protocol

The effects of esomeprazole on OE33, SK-GT-4, and OACM5.1C invasiveness were tested using 100,000 cells per well by the quantitative CytoSelectTM 96-well Cell Invasion Assay (Cell Biolabs, San Diego, USA) following the manufacturer's manual. In brief, cells were seeded in serum-free medium in the upper chamber of a modified Boyden chamber coated with a uniform layer of dried membrane matrix solution and allowed to invade toward 10% FBS for 24 h. Invasive cells that were able to degrade the matrix proteins in the layer, and ultimately pass through the pores on the bottom side of the membrane were stained and quantified as mean relative fluorescence units (RFUs) of repeated experiments (n = 7) measured at 480/520 nm using the Synergy HT plate reader (Biotek, Winooski, USA).

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