8 µm Chemotaxis Assays, 96-Well Format

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Cover the plate and transfer to a cell culture incubator for 24 hours.	Read the fluorescence with a fluorescence plate reader at 480 nm/520 nm.

Protocol tips
Cover the plate and transfer to a cell culture incubator for 24 hours.
Downstream tips
Read the fluorescence with a fluorescence plate reader at 480 nm/520 nm.

Publication protocol

CytoSelect 96-well cell migration assay (Cell Biolabs CBA-106) and CytoSelect 96-well cell invasion assay (Cell Biolabs CBA-112-COL) were performed according to the manufacturer's protocol. In short, cells were suspended in low-serum (0.5% fetal bovine serum) DMEM medium, and added to the top chambers of the 96-well cell migration plates or the collagen-coated 96-well cell invasion plates at a density of 50 k cells per well. Complete media was added to the bottom chambers as attractant. Twenty-four hours after incubation, migrating/invading cells were detached from the underside of the membrane using cell detachment solution, lysed with lysis buffer and stained with CyQuant GR dye solution. Fluorescence intensity was determined with Envision plate reader at 485/535 nm. Five biological replicates of the experiments were performed.

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Manufacturer protocol

Download the product protocol from Cell Biolabs for 8 µm Chemotaxis Assays, 96-Well Format below.

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