8 µm Chemotaxis Assays, 24-Well Format

Cell migration / Invasion cell type - FTC236

Experiment
Cell migration / Invasion cell type - FTC236
Product
8 µm Chemotaxis Assays, 24-Well Format from Cell Biolabs
Manufacturer
Cell Biolabs

Protocol tips

Protocol tips
Incubate for 4-6 hours in a cell culture incubator

Publication protocol

The experiment was carried out using the CytoSelect cell migration assay kit (Cell Biolabs, Inc., San Diego, CA) containing polycarbonate membrane inserts (8 μm pore size) in a 24-well plate. In brief, appropriate amount of cells were suspended in serum-free medium and added to the insert. The lower well was loaded with routine medium (with 10% FBS). After an incubation of 4-6 hours at 37°C in a humidified incubator with 5% CO2, non-migratory cells on the upper surface of the membrane were gently removed by wiping with a cotton swab. The cells that migrated through the pores and to the underside of the membrane were fixed, stained and photographed under a microscope. The number of cells was counted and averaged using three randomly selected areas per membrane. Doxycycline (0.8 µg/mL) was added to medium/cells over the time course of the desired experimental groups

Full paper   Login or join for free to view the full paper.

Reviews

8 µm Chemotaxis Assays, 24-Well Format from Cell Biolabs has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Start your discussion

Share your thoughts or question with experts in your field

Start a discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Cell migration / Invasion cell type - FTC236 using 8 µm Chemotaxis Assays, 24-Well Format from Cell Biolabs.

View full paper   Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from Cell Biolabs for 8 µm Chemotaxis Assays, 24-Well Format below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Cell migration / Invasion cell type - FTC236 using 8 µm Chemotaxis Assays, 24-Well Format from Cell Biolabs. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

  Outsource experiment
Become shareholder Discussions About us Contact Privacy Terms