JetFlex™ Genomic DNA Purification Kit

DNA isolation / purification Bacteria - Gram positive Actinomycytes

Experiment
DNA isolation / purification Bacteria - Gram positive Actinomycytes
Product
JetFlex™ Genomic DNA Purification Kit from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Upstream tips
The Proteinase K digestion is necessary if the blood sample is partially or completely blotted.

Publication protocol

Strain NA-134T, DSM 44106, was grown in DSMZ medium 3 (Azotobacter Medium) [44] at 28°C. DNA was isolated from 0.5-1 g of cell paste using Jetflex Genomic DNA Purification Kit (GENOMED 600100) following the standard protocol as recommended by the manufacturer with the following modifications: extended cell lysis time (60 min.) with additional 30µl achromopeptidase, lysostaphin, mutanolysin; proteinase K was applied in 6-fold the supplier recommended amount for 60 min. at 58°C. The purity, quality and size of the bulk gDNA preparation were according to DOE-JGI guidelines and routine protocols by the DNA Bank Network [45]. DNA is available through the DNA Bank Network [46].

Full paper   Login or join for free to view the full paper.

Reviews

JetFlex™ Genomic DNA Purification Kit from Thermo Fisher Scientific has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Discussion

5 years ago

Author: Israel

How can I improve my DNA yield?

The DNA concentration after using this DNA isolation kit is sometimes too low and thus it is not sufficient for my follow-up experiments. How can I improve it?

Discussion

5 years ago

Author: Milena Alexeyeva Russian Federation

Tips on storing DNA templates?

Hello there! I just started doing experiments on bacterial DNA and I would like your opinion on storing DNA templates. Which are the desired and most optimal conditions?

Share your thoughts or question with experts in your field by adding a discussion!

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA isolation / purification Bacteria - Gram positive Actinomycytes using JetFlex™ Genomic DNA Purification Kit from Thermo Fisher Scientific.

View full paper   Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for JetFlex™ Genomic DNA Purification Kit below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing DNA isolation / purification Bacteria - Gram positive Actinomycytes using JetFlex™ Genomic DNA Purification Kit from Thermo Fisher Scientific. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

  Outsource experiment
Become shareholder Discussions About us Contact Privacy Terms