QIAamp DNA Mini Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
		- Include RNAse treatment for 15-20 min. - Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C - Use prewarmed TE buffer to elute the DNA

Downstream tips
- Include RNAse treatment for 15-20 min. - Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C - Use prewarmed TE buffer to elute the DNA

Publication protocol

Preparation of nucleic acids
Genomic DNA was isolated using the QIAamp DNA Mini Kit (Qiagen). RNA was isolated using the RNeasy Mini Kit (Qiagen) according to the manufacturer’s protocol. Additional treatment with the TURBO DNA-free Kit (Ambion) was performed to eliminate traces of DNA. RNA integrity was assessed by visualization of sharp 28S and 18S rRNA bands on a 1% agarose gel.

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Manufacturer protocol

Download the product protocol from Qiagen for QIAamp DNA Mini Kit below.

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