NucleoSpin® Gel and PCR Clean-up

Protocol tips

Upstream tips	Protocol tips	Downstream tips
The amount of agarose excised from the gel should be as small as possible.	Remove the flow-through by aspiration. Avoid contamination of the collection tube rim.

Upstream tips
The amount of agarose excised from the gel should be as small as possible.
Protocol tips
Remove the flow-through by aspiration. Avoid contamination of the collection tube rim.

Publication protocol

CfDNA was extracted with the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel, Düren, Germany; #1502/001), according to the instructions described by the PCR clean-up user manual. Briefly, samples were removed from the −80 °C freezer and thawed at 37 °C and then vortexed and centrifuged briefly. For each biological replicate, cfDNA was extracted in triplicate. For every sample, 600 µL of growth medium was mixed with 1200 µL of binding buffer. Samples were then vortexed, the entire volume of growth media was added to the spin column in small regiments, and centrifuged at 11 000×g for 1 min at room temperature. The columns were then washed twice, followed by the elution of cfDNA into 20 µL of elution buffer.

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Manufacturer protocol

Download the product protocol from Macherey Nagel for NucleoSpin® Gel and PCR Clean-up below.

Download manufacturer protocol

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