Atg3 Antibody

Autophagy assay cell type - KG1 cells (bone marrow myelogenous leukaemia)

Experiment
Autophagy assay cell type - KG1 cells (bone marrow myelogenous leukaemia)
Product
Atg3 Antibody from Cell Signaling Technology
Manufacturer
Cell Signaling Technology

Protocol tips

Protocol tips
Dilute Ab at 1:100 and incubate overnight at 4 °C

Publication protocol

Immunoblotting was carried out as described previously5. Proteins were separated on SDS-PAGE gels, electro-transferred onto polyvinylidene difluoride membranes and incubated overnight at 4 °C with the following antibodies: anti-Atg3, anti-Atg5 (D1G9), anti-Atg7 (D12B11), anti-Beclin (D40C5), anti-caspase-3 (Cell Signaling), anti-Bcl-2 (Millipore), anti-Bax (Millipore), anti-LC3 (Sigma-Aldrich), anti-LXRα (SantaCruz), anti-LXRβ (SantaCruz), anti-Nur77 (Active motif), anti-NOR1 (R&D systems), anti-PARP (Cell Signaling), anti-Vps34 (Cell Signaling), or anti-Actin (Millipore). For visualization, an ECL plus kit (Amersham Biosciences) was used, and chemiluminescence was measured by autoradiography (Supplementary Fig. 9). Specific bands were quantified with ImageQuant software.

Full paper   Login or join for free to view the full paper.

Reviews

Atg3 Antibody from Cell Signaling Technology has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Start your discussion

Share your thoughts or question with experts in your field

Start a discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Autophagy assay cell type - KG1 cells (bone marrow myelogenous leukaemia) using Atg3 Antibody from Cell Signaling Technology.

View full paper   Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from Cell Signaling Technology for Atg3 Antibody below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Autophagy assay cell type - KG1 cells (bone marrow myelogenous leukaemia) using Atg3 Antibody from Cell Signaling Technology. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Become shareholder Discussions About us Contact Privacy Terms