Anti-LC3B antibody produced in rabbit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Protein lysates were separated by SDS-PAGE and probed with the following antibodies	Relative levels of protein to ACTB loading control were quantified by densitometry using ImageJ, and normalized to the lane with the highest signal.

Protocol tips
Protein lysates were separated by SDS-PAGE and probed with the following antibodies
Downstream tips
Relative levels of protein to ACTB loading control were quantified by densitometry using ImageJ, and normalized to the lane with the highest signal.

Publication protocol

Protein lysates were separated by SDS-PAGE and probed with the following antibodies: SQSTM1/p62 (Abnova, H00008878-M01), BCL2/B-cell CLL/lymphoma 2 (Santa Cruz, sc-492), BECN1/Beclin 1 (Santa Cruz, sc-11427), pro-CASP3/caspase 3 (Santa Cruz, sc-7148), cleaved CASP3/caspase 3 (Cell Signaling, 9661), BAX/BCL2-associated X protein (Santa Cruz, sc-526), LAMP1/Lysosomal-associated membrane protein 1 (Developmental Studies, H4A3), HSPA5/GRP78 (Santa Cruz, sc-1050), ATG7 (Abcam, ab133528), microtubule-associated protein 1 light chain 3 α/LC3A (Sigma, L8918), ACTB/β-actin (Sigma, A5441), and CTSD/cathepsin D (Santa Cruz, sc-6486). Relative levels of protein to ACTB loading control were quantified by densitometry using ImageJ, and normalized to the lane with the highest signal.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Autophagy assay cell type - SH-SY5Y using Anti-LC3B antibody produced in rabbit from Sigma-Aldrich.

Manufacturer protocol

Download the product protocol from Sigma-Aldrich for Anti-LC3B antibody produced in rabbit below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Autophagy assay cell type - SH-SY5Y using Anti-LC3B antibody produced in rabbit from Sigma-Aldrich. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.