Atg7 Antibody

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Dilute primary Ab at 1:1000 and incubate overnight at 4°C.

Protocol tips
Dilute primary Ab at 1:1000 and incubate overnight at 4°C.

Publication protocol

After transfection and/or treatment with chemicals, cells were lysed for the western blot assay as described previously52. Bands were incubated with primary antibodies against PARP(SC-7150, Santa Cruz Biotechnology), glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (SC-47724, Santa Cruz Biotechnology), β-actin(sc-47778, Santa Cruz Biotechnology), ARRB1 (ab32099, Abcam), ARRB2 (10171-1-AP, Proteintech), LC3B (NB100-2220), GRP78 (NBP1-06274), eIF2α(NB100-81896), and phosphor-eIF2α(NB110-56949) were purchased from Novus Biologicals, phosphor-p44/42MAPK(T202/Y204) (#4370S), phosphor-MEK(#9121S), phosphor-AKT(T308) (#9275S), ATG7 (#2631S), and Beclin-1 (#3738S) were purchased from Cell Signaling Technology.

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Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Autophagy assay cell type - SH-SY5Y using Atg7 Antibody from Cell Signaling Technology.

Manufacturer protocol

Download the product protocol from Cell Signaling Technology for Atg7 Antibody below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Autophagy assay cell type - SH-SY5Y using Atg7 Antibody from Cell Signaling Technology. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.