mTOR (7C10) Rabbit mAb

Protocol tips

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	Dilute primary Ab at 1:1000 and incubate overnight at 4°C

Protocol tips
Dilute primary Ab at 1:1000 and incubate overnight at 4°C

Publication protocol

The immunoblotting was performed as previously described,82 using rabbit antibodies (Cell Signaling Technology) against LC3B (2775), BECN1/Beclin 1 (3495), PRKAA/AMPKA (2603), phospho-PRKAA/AMPKA (Thr172; 2535), AKT1 (9272), phospho-AKT1 (Ser473; 4058), MTOR (2983), phospho-MTOR (Ser2448; 2971), RPS6KB1/S6K1 (2708), phospho-RPS6KB1/S6K1 (Thr389; 9205), COX4I1/COX IV (4850), and actin (4968) as primary antibodies, and peroxidase-conjugated goat anti-rabbit IgG (Jackson IP Laboratories, 111-035-003) as a secondary antibody. The levels of LC3B-II were quantified by densitometry using ImageJ software and expressed relative to actin. The results are presented as the fold change in signal intensity compared to that of the untreated control, which was arbitrarily set to 1.

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Manufacturer protocol

Download the product protocol from Cell Signaling Technology for mTOR (7C10) Rabbit mAb below.

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