Anti-Apg7 antibody [EPR6251]

Protocol tips

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	Incubate the membrane with diluted primary antibody in blocking buffer and incubate overnight at 4C

Protocol tips
Incubate the membrane with diluted primary antibody in blocking buffer and incubate overnight at 4C

Publication protocol

Protein lysates were separated by SDS-PAGE and probed with the following antibodies: SQSTM1/p62 (Abnova, H00008878-M01), BCL2/B-cell CLL/lymphoma 2 (Santa Cruz, sc-492), BECN1/Beclin 1 (Santa Cruz, sc-11427), pro-CASP3/caspase 3 (Santa Cruz, sc-7148), cleaved CASP3/caspase 3 (Cell Signaling, 9661), BAX/BCL2-associated X protein (Santa Cruz, sc-526), LAMP1/Lysosomal-associated membrane protein 1 (Developmental Studies, H4A3), HSPA5/GRP78 (Santa Cruz, sc-1050), ATG7 (Abcam, ab133528), microtubule-associated protein 1 light chain 3 α/LC3A (Sigma, L8918), ACTB/β-actin (Sigma, A5441), and CTSD/cathepsin D (Santa Cruz, sc-6486). Relative levels of protein to ACTB loading control were quantified by densitometry using ImageJ, and normalized to the lane with the highest signal.

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Papers

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Manufacturer protocol

Download the product protocol from Abcam for Anti-Apg7 antibody [EPR6251] below.

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