APG5 Antibody (C-1)

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	Immunoprecipitation (IP) and immunoblotting (IB) were performed as previously described [42]. In some cases, membranes were stripped and re-probed with specific antibodies. To quantify changes, the densitometries of protein bands were determined with a calibrated GS-670 densitometer. For endogenous interactions, HeLa cells grown in 10 cm2 dishes were harvested and the cell lysates were then subjected to IP. All IP and IB experiments were performed as three independent experiments.

Protocol tips

Immunoprecipitation (IP) and immunoblotting (IB) were performed as previously described [42]. In some cases, membranes were stripped and re-probed with specific antibodies. To quantify changes, the densitometries of protein bands were determined with a calibrated GS-670 densitometer. For endogenous interactions, HeLa cells grown in 10 cm2 dishes were harvested and the cell lysates were then subjected to IP. All IP and IB experiments were performed as three independent experiments.

Publication protocol

Immunoprecipitation (IP) and immunoblotting (IB) were performed as previously described [42]. In some cases, membranes were stripped and re-probed with specific antibodies. To quantify changes, the densitometries of protein bands were determined with a calibrated GS-670 densitometer. For endogenous interactions, HeLa cells grown in 10 cm2 dishes were harvested and the cell lysates were then subjected to IP. All IP and IB experiments were performed as three independent experiments.

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