Lipofectamine® RNAiMAX Transfection Reagent

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	siRNA concentration- 30 nM Add diluted siRNA to diluted Lipofectamine® RNAiMAX Reagent (1:1 ratio).  Incubate for 5 minutes at room temperature and add to cells.  Incubate cells for 3 days at 37°C.

Protocol tips
siRNA concentration- 30 nM Add diluted siRNA to diluted Lipofectamine® RNAiMAX Reagent (1:1 ratio).  Incubate for 5 minutes at room temperature and add to cells.  Incubate cells for 3 days at 37°C.

Publication protocol

siGAPDH-loaded YSK12-MEND and siGAPDH-loaded RNAiMAX were incubated at a siRNA concentration of 30 nM in OPTI-MEM I at 37 °C, 5% CO2, which was the same condition as siRNA transfection experiment. The RNAiMAX preparation was carried out following the manufacturer’s instructions. After 0.5, 1, 2, and 6 h incubation, the diameters were measured with a ZETASIZER Nano.

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for Lipofectamine® RNAiMAX Transfection Reagent below.

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