Anti-mTOR antibody [53E11]

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Use a concentration of 1 - 5 µg/ml.

Protocol tips
Use a concentration of 1 - 5 µg/ml.

Publication protocol

Western blot analyses were performed as described previously[19]. Rabbit antibodies against LC3 (monoclonal ab192890), SQSTM1/p62 (monoclonal ab109012), p-mTOR (phosphor-S2481) (monoclonal ab137133), mTOR (monoclonal ab87540), p-PI3K (phosphor-Y607) (polyclonal ab182651), PI3K (monoclonal ab40755) and actin (polyclonal ab8227) were purchased from Abcam (Cambridge, MA, United States).

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Manufacturer protocol

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