Anti-PI 3 Kinase p85 alpha antibody [EP380Y]

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Dilute primary Ab between 1:500 - 1:1000 and incubate overnight at 4°C

Protocol tips
Dilute primary Ab between 1:500 - 1:1000 and incubate overnight at 4°C

Publication protocol

Western blot analyses were performed as described previously[19]. Rabbit antibodies against LC3 (monoclonal ab192890), SQSTM1/p62 (monoclonal ab109012), p-mTOR (phosphor-S2481) (monoclonal ab137133), mTOR (monoclonal ab87540), p-PI3K (phosphor-Y607) (polyclonal ab182651), PI3K (monoclonal ab40755) and actin (polyclonal ab8227) were purchased from Abcam (Cambridge, MA, United States).

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Manufacturer protocol

Download the product protocol from Abcam for Anti-PI 3 Kinase p85 alpha antibody [EP380Y] below.

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