Lipofectamine® RNAiMAX Transfection Reagent
siRNA / RNAi /miRNA transfection Human Cells - NK-92 Lipofectamine
Protocol tips
| Protocol tips |
|---|
| Add diluted siRNA to diluted Lipofectamine® RNAiMAX Reagent (1:1 ratio). Incubate for 5 min at RT. Incubate cells for 24-72 h at 37°C. |
Publication protocol
Silencer select validated Bid, p53, and control small interfering RNAs (siRNAs) were purchased from Life Technologies. Subconfluent cells were transfected with siRNA using Lipofectamine RNAiMAX (Life Technologies) in Opti-MEM according to the manufacturer’s instructions.Full paper Login or join for free to view the full paper.
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Discussion
Discussion
5 years ago
Author:
Keith L. Morrison
siRNA/RNAi/miRNA transfection human
I would like to regulate the expression of a gene and in order to do that, I have purchased specific siRNA. After optimizing my transfection protocol and using electroporation I have achieved a 60-70% reduction of the gene of interest. However, I cannot observe a significant reduction of mRNA expression but only a reduction of protein. What might be the problem? Could the problem be in my cell treatment method?
Papers
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Manufacturer protocol
Download the product protocol from Thermo Fisher Scientific for Lipofectamine® RNAiMAX Transfection Reagent below.
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