Anti-PI3-kinase p85-α antibody produced in rabbit

Protocol tips

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	Dilute primary Ab at 1:1000 and incubate overnight at 4°C

Protocol tips
Dilute primary Ab at 1:1000 and incubate overnight at 4°C

Publication protocol

Western blotting was conducted with the following primary antibodies (all diluted 1:1000): anti-CCR10 (#sc-365957, Santa Cruz Biotechnology, Santa Cruz, CA, USA), anti-GFP (#sc-8334, Santa Cruz Biotechnology), anti-TNF (#3707, Cell Signaling Technology, Danvers, MA, USA), anti-PI3K (#SAB4502195, Sigma-Aldrich, St Louis, MO, USA),anti-Akt (#9272, Cell Signaling Technology), anti-phospho-AktSer473 (p-Akt) (#4060, Cell Signaling Technology), anti-cleaved caspase-3 (#9661, Cell Signaling Technology), anti-cleaved PARP (#9546, Cell Signaling Technology), anti-PCNA (#sc-56, Santa Cruz Biotechnology), and β-actin (#5125, Cell Signaling Technology). Bands were detected with ECL reagents (KeyGen Biotech, Nanjing, China). Band signals were quantified through densitometry. Results were reported as the ratio of the target protein’s densitometry units to β-actin’s densitometry units.

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Manufacturer protocol

Download the product protocol from Sigma-Aldrich for Anti-PI3-kinase p85-α antibody produced in rabbit below.

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