TransIT®-LT1 Transfection Reagent

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Incubate TransIT-LT1 Reagent:siRNA complexe mixture at room temperature for 15–30 minutes to allow sufficient time for complexes to form and add to cells.  Incubate for 24-48 hours.

Protocol tips
Incubate TransIT-LT1 Reagent:siRNA complexe mixture at room temperature for 15–30 minutes to allow sufficient time for complexes to form and add to cells.  Incubate for 24-48 hours.

Publication protocol

GFP-conjugated (pCMV6-AC-GFP) empty or CBR1 plasmids were purchased from Origene (Rockville, MD, USA). The siRNAs were purchased from IDT (Cambridge, MA, USA). Cells were transfected with plasmid or siRNA using TransIT-LT1 or TransIT-TKO Transfection Reagent (Mirus Bio, Madison, WI, USA) according to the manufacturer’s recommendations

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing siRNA / RNAi /miRNA transfection Human Cells - HNSCC Polymer / Lipid using TransIT®-LT1 Transfection Reagent from Mirus.

Manufacturer protocol

Download the product protocol from Mirus for TransIT®-LT1 Transfection Reagent below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing siRNA / RNAi /miRNA transfection Human Cells - HNSCC Polymer / Lipid using TransIT®-LT1 Transfection Reagent from Mirus. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.