ROS-ID® Total ROS/Superoxide detection kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- 100,000 cells per well were seeded in a 12 well plate	- Cells were treated with 500 μl of ROS/Superoxide Detection Mix for 30 minutes at 37°C

Upstream tips
- 100,000 cells per well were seeded in a 12 well plate
Protocol tips
- Cells were treated with 500 μl of ROS/Superoxide Detection Mix for 30 minutes at 37°C

Publication protocol

The ROS and superoxide levels were measured using a commercial kit according to the manufacturer's instructions (Total ROS/Superoxide Detection Kit; Enzo Life Sciences). In brief, A549 cells were plated in 12-well plates at 100,000 cells per well and cultured for 24 h. Cells were then treated with 10 mM stock solutions of drug dissolved in DMSO at varying concentrations and incubation times. After incubation, cells were stained with 500 μl of ROS/Superoxide Detection Mix for 30 minutes at 37°C, and analyzed by using flow cytometry.

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Manufacturer protocol

Download the product protocol from Enzo Life Sciences for ROS-ID® Total ROS/Superoxide detection kit below.

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