MitoSOX™ Red Mitochondrial Superoxide Indicator, for live-cell imaging

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- Cells were pretreated with LGH00168 for 1 h.	- After washing step, cells were incubated with 5 μmol/L of MitoSOX for 20 min in the dark.

Upstream tips
- Cells were pretreated with LGH00168 for 1 h.
Protocol tips
- After washing step, cells were incubated with 5 μmol/L of MitoSOX for 20 min in the dark.

Publication protocol

The mito-ROS level was examined by a MitoSOX Red mitochondrial superoxide indicator (Cat #M36008, Life technologies, Grand Island, NY, USA) according to the manufacturer's instructions. Briefly, cells were treated with LGH00168 for 1 h, collected by trypsin treatment, washed twice with ice-cold 1× PBS, and then incubated with 5 μmol/L of MitoSOX for 20 min in the dark. The mito-ROS level was measured using a BD FACSCalibur Flow Cytometer and analyzed with FCS Express V3 software (De Novo Software, Glendale, CA, USA).

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for MitoSOX™ Red Mitochondrial Superoxide Indicator, for live-cell imaging below.

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