ROS-ID® Total ROS/Superoxide detection kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- 1 × 10 ^5 cells were seeded.	-positive control cells were incubated for 30 min at 37 °C in dark

Upstream tips
- 1 × 10 ^5 cells were seeded.
Protocol tips
-positive control cells were incubated for 30 min at 37 °C in dark

Publication protocol

Change in ROS level was detected using the Total ROS/Superoxide Detection Kit (ENZO Life Sciences, Farmingdale, NY, USA). Approximately 1 × 105 of treated cells were washed with 1 × wash buffer, re-suspended in 500 µL of Oxidative Stress Detection Reagent and incubated for 30 min at 37 °C in dark. Cells were then washed with 1 × wash buffer and treated with compounds for different lengths of time. All treatments were performed under normal tissue culture conditions. After incubation, cells were washed with 1 × wash buffer once and re-suspended in 500 µL of 1 × wash buffer before being analyzed by flow cytometry. Pyocyanin-treated cells were used as positive control cells.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing ROS assay cell type - K562 human leukemia cells using ROS-ID® Total ROS/Superoxide detection kit from Enzo Life Sciences.

Manufacturer protocol

Download the product protocol from Enzo Life Sciences for ROS-ID® Total ROS/Superoxide detection kit below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing ROS assay cell type - K562 human leukemia cells using ROS-ID® Total ROS/Superoxide detection kit from Enzo Life Sciences. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.