DCFDA - Cellular Reactive Oxygen Species Detection Assay Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- 1.5×105 cells/well in 96-well plates. - Cells were treated with 20 µM BL or liposomal BL for 48 h.	- 20 µM of DCFDA was added to cells and incubated for 37°C for further 30 min in the dark.

Upstream tips
- 1.5×105 cells/well in 96-well plates. - Cells were treated with 20 µM BL or liposomal BL for 48 h.
Protocol tips
- 20 µM of DCFDA was added to cells and incubated for 37°C for further 30 min in the dark.

Publication protocol

The measurement of intracellular ROS was determined by the Cellular Reactive Oxygen Species Detection Assay kit (Abcam, Cambridge, UK), which uses the cell permeant reagent 2′,7′-dichlorofluorescin diacetate (DCFDA), a fluorogenic dye that measures ROS activity within the cell. Briefly, K562 cells were cultured in complete phenol-red free RPMI medium at a density of 1.5×105 cells/well in 96-well plates and were treated with 20 µM BL or liposomal BL for 48 h. After the incubation time, the cell treatments were overlaid with 20 µM DCFDA (100 µl) and incubated at 37°C for further 30 min in the dark. The fluorescence of the treatments was detected at the excitation and emission spectra of 485 and 520 nm, respectively, by FLUOstar Omega (BMG Labtech).

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing ROS assay cell type - K562 human leukemia cells using DCFDA - Cellular Reactive Oxygen Species Detection Assay Kit from Abcam.

Manufacturer protocol

Download the product protocol from Abcam for DCFDA - Cellular Reactive Oxygen Species Detection Assay Kit below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing ROS assay cell type - K562 human leukemia cells using DCFDA - Cellular Reactive Oxygen Species Detection Assay Kit from Abcam. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.