ROS-ID® Total ROS/Superoxide detection kit

Protocol tips

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	- Cells were stained and incubated for 30 min. at 37°C in the dark.

Protocol tips
- Cells were stained and incubated for 30 min. at 37°C in the dark.

Publication protocol

Cells transiently transfected with 1 μg of pCMV6-Entry or pCMV6-SOD3 were loaded with ROS/Superoxide Detection Mix of Total ROS/Superoxide Detection Kit (Enzo Life Sciences, Inc.) at 37°C incubator for 1 h. The Mix was removed and washed with 1× Wash Buffer. They were observed under a fluorescence/confocal microscope using standard excitation/emission filter sets.

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Manufacturer protocol

Download the product protocol from Enzo Life Sciences for ROS-ID® Total ROS/Superoxide detection kit below.

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