OxiSelect™ Intracellular ROS Assay Kit (Green Fluorescence)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- 2.5 × 105 were seeded.	- Treated with DCFH-DA for 1 h at 37°C. - After DCFH-DA treatement, cells were treated with Vitisin A (4 µM) and/or TRAIL (20 ng/ml) for 1 h at 37°C

Upstream tips
- 2.5 × 105 were seeded.
Protocol tips
- Treated with DCFH-DA for 1 h at 37°C. - After DCFH-DA treatement, cells were treated with Vitisin A (4 µM) and/or TRAIL (20 ng/ml) for 1 h at 37°C

Publication protocol

PC-3 cells (2.5 × 105) were cultured in a 96-well cell culture plate and then pre-incubated with DCFH-DA for 1 h at 37°C. After incubation, DCFH-DA-loaded cells were treated with Vitisin A (4 µM) and/or TRAIL (20 ng/ml) for 1 h at 37°C. Intracellular levels of ROS were measured by flow cytometry using 2070-diacetyl dichlorofluorescein (DCFDA). ROS production was analyzed using the CellQuest Software with a FACSCalibur flow cytometer. Also, the concentration of DCF was quantified using the OxiSelect Intracellular ROS Assay Kit (Cell Biolabs, CA, USA) and a Fluoroskan Ascent microplate fluorometer (Thomas Scientific, NJ, USA).

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Manufacturer protocol

Download the product protocol from Cell Biolabs for OxiSelect™ Intracellular ROS Assay Kit (Green Fluorescence) below.

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