MitoSOX™ Red Mitochondrial Superoxide Indicator, for live-cell imaging

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- 5×104 cells/well were seeded in 6 well plate. - Cells were pretreated with DPT at different time intervals.	- Cells were incubated with DCFH-DA or MitoSOX at a final concentration of 10 μM or 5 μM for 15 min at 37°C

Upstream tips
- 5×104 cells/well were seeded in 6 well plate. - Cells were pretreated with DPT at different time intervals.
Protocol tips
- Cells were incubated with DCFH-DA or MitoSOX at a final concentration of 10 μM or 5 μM for 15 min at 37°C

Publication protocol

Total intracellular ROS and mitochondrial ROS levels were measured by DCFH-DA and MitoSOX, respectively. Human prostate cancer PC-3 cells were cultured in a 6-well plates at a density of 5×104 cells/well. After exposure to DPT for the indicated times, the cells were incubated with DCFH-DA or MitoSOX at a final concentration of 10 μM or 5 μM for 15 min at 37°C, respectively. Cells were washed twice with PBS and fluorescence positive cells were measured with flow cytometry and analyzed with CellQuest analysis software.

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for MitoSOX™ Red Mitochondrial Superoxide Indicator, for live-cell imaging below.

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