OxiSelect™ In Vitro ROS/RNS Assay Kit (Green Fluorescence)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
		- Due to light-induced auto-oxidation, the stock DCF-DiOxyQ solution and all subsequent DCF-DiOxy and DCFH solutions must be protected from light.

Downstream tips
- Due to light-induced auto-oxidation, the stock DCF-DiOxyQ solution and all subsequent DCF-DiOxy and DCFH solutions must be protected from light.

Publication protocol

OxiSelect Intracellular ROS assay kit (Cell Biolabs, Inc., San Diego, CA) was used according to the manufacturer’s instructions. The treated cells were lysed and the amount of intracellular ROS was estimated from dichlorodihydrofluorescein (DCF) production measured by flow cytometry or a fluorometric plate reader (FLUOstar OPTIMA, BMG Labtechnologies, Cary, NC, USA) using excitation and emission wavelengths of 480 nm/530 nm.

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Manufacturer protocol

Download the product protocol from Cell Biolabs for OxiSelect™ In Vitro ROS/RNS Assay Kit (Green Fluorescence) below.

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