Corning® BioCoat™ Matrigel® Invasion Chambers with 8.0 µm PET Membrane in two 24-well Plates

Protocol tips

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	Incubate the Chambers for 24 hours in a humidified tissue culture incubator, at 37C, 5% CO2 atmosphere.

Protocol tips
Incubate the Chambers for 24 hours in a humidified tissue culture incubator, at 37C, 5% CO2 atmosphere.

Publication protocol

Invasive ability was measured by using 24-well BioCoat cell culture inserts (Costar, Corning, NY) with an 8-μm-porosity polyethylene terephthalate membrane coated with Matrigel Basement Membrane Matrix (Trevigen, Gaithersburg, MD). In brief, the Matrigel was allowed to rehydrate for 2 h at room temperature by adding warm, serum-free RPMI 1640. The wells of the lower chamber were filled with medium containing 10% fetal bovine serum. Five x 104 cells/well were seeded in the upper compartment in serum-free medium. The invasion assay was done at 37°C in a 5% CO2 humidified incubator for 24 h. At the end of the invasion assay, filters were removed, fixed with Methyl alcohol and stained with the hematoxylin. Cells on the upper surface of the filters were removed by wiping with a cotton swab and invasion was determined by counting the cells that migrated to the lower side of the filter using a microscope at x400 magnification. All experiments were performed in triplicates

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Manufacturer protocol

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