Protocol tips
Upstream tips |
Protocol tips |
Downstream tips |
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Starve cells by incubating 18-24 hours prior to assay in appropriate serumfree medium (DMEM, EMEM, or equivalent).
Cover plate and incubate for 24 - 72 hours at 37°C in a CO2 incubator (4-6% CO2). |
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Protocol tips |
Starve cells by incubating 18-24 hours prior to assay in appropriate serumfree medium (DMEM, EMEM, or equivalent).
Cover plate and incubate for 24 - 72 hours at 37°C in a CO2 incubator (4-6% CO2). |
Publication protocol
In vitro cell invasion assays were performed with the QCM ECMatrix 24-well (8 µM) Fluorimetric Cell Invasion Assay kit (Chemicon; Millipore) according to the manufacturer's instructions , . The kit used an insert polycarbonate membrane with an 8 µM pore size. The insert in the invasion kit was coated with a thin layer of ECMatrix. Cell invasion was determined by fluorescence. Each experiment was repeated for three times in duplicates.
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Papers
Check out relevant papers found by Labettor's AI that are relevant for performing Cell migration / Invasion cell type - MG-63 using QCM ECMatrix Cell Invasion Assay, 24-well (8 µm), fluorimetric from Merck Millipore.
Paper title
Endothelin A Receptor Antagonism Enhances Inhibitory Effects of Anti-Ganglioside GD2 Monoclonal Antibody on Invasiveness and Viability of Human Osteosarcoma Cells
Manufacturer protocol
Download the product protocol from Merck Millipore for QCM ECMatrix Cell Invasion Assay, 24-well (8 µm), fluorimetric below.
Download manufacturer protocol
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