QCM ECMatrix Cell Invasion Assay, 24-well (8 µm), fluorimetric

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Starve cells by incubating 18-24 hours prior to assay in appropriate serumfree medium (DMEM, EMEM, or equivalent). Cover plate and incubate for 24 - 72 hours at 37°C in a CO2 incubator (4-6% CO2).

Protocol tips
Starve cells by incubating 18-24 hours prior to assay in appropriate serumfree medium (DMEM, EMEM, or equivalent). Cover plate and incubate for 24 - 72 hours at 37°C in a CO2 incubator (4-6% CO2).

Publication protocol

In vitro cell invasion assays were performed with the QCM ECMatrix 24-well (8 µM) Fluorimetric Cell Invasion Assay kit (Chemicon; Millipore) according to the manufacturer's instructions , . The kit used an insert polycarbonate membrane with an 8 µM pore size. The insert in the invasion kit was coated with a thin layer of ECMatrix. Cell invasion was determined by fluorescence. Each experiment was repeated for three times in duplicates.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Cell migration / Invasion cell type - MG-63 using QCM ECMatrix Cell Invasion Assay, 24-well (8 µm), fluorimetric from Merck Millipore.

Manufacturer protocol

Download the product protocol from Merck Millipore for QCM ECMatrix Cell Invasion Assay, 24-well (8 µm), fluorimetric below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Cell migration / Invasion cell type - MG-63 using QCM ECMatrix Cell Invasion Assay, 24-well (8 µm), fluorimetric from Merck Millipore. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.