Cultrex 3D Culture BME Cell Invasion Assay, 96 well

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Incubate at 37 °C in a tissue culture incubator for 72 hours to promote spheroid formation.	Incubate at 37 °C in a tissue culture incubator for 6 days, and photograph the spheroid in each well every 24 hours using the 4X objective

Protocol tips
Incubate at 37 °C in a tissue culture incubator for 72 hours to promote spheroid formation.
Downstream tips
Incubate at 37 °C in a tissue culture incubator for 6 days, and photograph the spheroid in each well every 24 hours using the 4X objective

Publication protocol

Migration and Invasion were performed using Cultrex96 well 3D Spheroid BME Cell Invasion Assay kit (Amsbio, MA, USA). For the migration assay; all the three cell lines (AsPC-1, BxPC-3, MIA PaCA-2) harvested, count (5 × 103 cells), and seeded in 3D culture qualified 96 well spheroid formation plate and placed in culture incubator for 72 hrs at 37 °C to promote spheroid formation. After 72 hrs, cells were incubated with invasion matrix to each well of the 3D culture plate and treated with CCL5 and the combination of CCL5 and inhibitor maraviroc followed by manufacturer protocol. Cells containing invasion-modulating compound were incubated for six (6) days at 37 °C. Images were captured in each well every 24 hrs using 10 × objectives of the microscope. The changes in the area of the invasive structures were analyzed using the Image-J software (NIH).

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Manufacturer protocol

Download the product protocol from Amsbio for Cultrex 3D Culture BME Cell Invasion Assay, 96 well below.

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