Corning® BioCoat™ Matrigel® Invasion Chambers with 8.0 µm PET Membrane in two 24-well Plates

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Add warm (37C) bicarbonate based culture medium to the interior of the inserts and bottom of wells. Allow to rehydrate for 2 hours in humidified tissue culture incubator, 37C, 5% CO2 atmosphere.	Incubate the Corning BioCoat™ Matrigel Invasion Chambers for 20 hours in a humidified tissue culture incubator, at 37o C, 5% CO2 atmosphere.

Upstream tips
Add warm (37C) bicarbonate based culture medium to the interior of the inserts and bottom of wells. Allow to rehydrate for 2 hours in humidified tissue culture incubator, 37C, 5% CO2 atmosphere.
Protocol tips
Incubate the Corning BioCoat™ Matrigel Invasion Chambers for 20 hours in a humidified tissue culture incubator, at 37o C, 5% CO2 atmosphere.

Publication protocol

The BD Biocoat invasion kit (BD) was used to evaluate the tumor invasive ability according to the manufacturer's protocol. Briefly, around 5×104 cells of PANC-1 and BxPC-3 with basal media were transferred in each 6-well upper chamber in the presence or absence of OPP. In the meantime, 3 mm of culture medium with 10% FBS was added into each lower chamber of a 24-well plate. After 20 h of incubation, the cells in the upper chamber were removed using a cotton stick. Later, the cells that invaded through the Matrigel matrix membrane were stained with 4 μg/ml Calcein AM in Hanks buffered saline at 37°C for 1 h. Each of the experimental conditions was performed in triplicates. Subsequently, fluorescence of the invaded cells was read in an ULTRA Multifunctional Microplate Reader (Tecan) at excitation/emission wavelengths of 530/590 nm. These fluorescently labeled invasive cells were also photographed under a fluorescent microscope.

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Manufacturer protocol

Download the product protocol from Corning for Corning® BioCoat™ Matrigel® Invasion Chambers with 8.0 µm PET Membrane in two 24-well Plates below.

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